Assessment of feline spermatozoa quality via MTT assay from post-orchidectomy epididymal salvage

Hussain, Nadia (2023) Assessment of feline spermatozoa quality via MTT assay from post-orchidectomy epididymal salvage. [Project Paper] (Submitted)

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Abstract

MTT (3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyltetrazolium bromide) assay is a colorimetric method to measure cellular metabolic activity of active sperm cells. This study aims to determine the correlation of semen quality via traditional semen evaluation and MTT assay as well as to compare two epididymal semen salvage techniques in domestic cats. Twenty left and right testicles were collected and epididymal semen were extracted via two different techniques of mincing and slicing. Semen evaluation was performed to assess wave pattern, motility, live percentage, abnormal sperm percentage and concentration. Subsequently, each sample underwent MTT assay at absorbance of 570nm. Using Spearman’s correlation coefficient analysis, there were positive correlation between MTT absorbance rates and motility (r= 0.061) and abnormal sperm count (r = 0.296) of the left testicles but the test was not significant (P > 0.05). All other parameters for the left testicle such as live sperm count (r = - 0.409), wave pattern (r = - 0.021) and total sperm count (r = - 0.201) had a negative correlation with MTT absorbance rate that was not significant (P > 0.05). The same test was repeated for the right testicle which resulted in positive correlation between MTT absorbance rates and abnormal sperm count (r = 0.396) which were not significant (P > 0.05). All other parameters such as motility (r = - 0.313), live sperm count (r = - 0.396), wave pattern (r = - 0.305) and total sperm count (r = - 0.370) had a negative correlation with MTT absorbance rate that was not significant (P > 0.05). Wilcoxon signed ranked test was performed to compare the two epididymal semen salvage techniques of, mincing and slicing. The results showed no significant (P > 0.05) difference between the techniques. The lack of significant correlations between the MTT assay and traditional sperm evaluation parameters raises concerns regarding the MTT assay's utility for measuring sperm quality. Further research is necessary to refine the MTT assay for more accurate assessments of sperm quality in domestic cats. Although there was no significant difference between the mincing and slicing techniques, the mincing technique was more convenient for sperm salvage.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	30 Oct 2024 07:58
Last Modified:	30 Oct 2024 07:58
URI:	http://psaspb.upm.edu.my/id/eprint/2106

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