Phan, Yong Wei (2023) Detection of African swine fever and classical swine fever viral antigen from formalin-fixed paraffin-embedded tissues of wild and domestic pigs. [Project Paper] (Submitted)
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Abstract
African swine fever (ASF) and classical swine fever (CSF) is caused by African swine fever virus (ASFV), a DNA virus belonging to the family of Asfaviridae and genus Asfivirus, and classical swine fever virus (CSFV), a RNA virus belonging to the Pestivirus genus within the Flaviviridae family respectively. ASF has spread throughout Asia since 2018 and first reported in Malaysia in February 2021, while CSF is endemic in few countries in Asia including Malaysia. Both diseases have negatively impacted the economy of Malaysia due to high mortality and high morbidity rate in domestic pigs. This study aimed to detect the ASF and CSF viral antigen in formalin-fixed paraffin embedded tissue (FFPE) samples by using immunohistochemistry (IHC) and to characterise the histopathological lesion caused by both viruses. Paraffin blocks and H&E slides of spleen, tonsil and lymph nodes of 17 domestic pigs and 3 wild boars are retrieved, and paraffin blocks are processed into IHC slides. For IHC, primary antibody of dilution of 1:1000 is used for ASF and dilution 1:100 is used for CSF. IHC revealed ASFV antigen positive cells in 1/20 (5%) samples and CSFV antigen positive cells in 4/30 (20%) samples. All CSF positive samples show lymphoid depletion and 3/4 (75%) shows hemorrhages. This study illustrates the need for farmers to strengthen the biosecurity to prevent transmission of ASF and CSF into the farm.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms. Nordeena Abdul Aziz |
| Date Deposited: | 15 Nov 2024 01:37 |
| Last Modified: | 15 Nov 2024 01:37 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2115 |
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