Shahril Agus, Syahir Aiman (2023) Effect of N, N-dimethylglycine (DMG) supplementation on haematological parameters and frequency of CD4+ and CD8+ T cells in cats post-vaccination. [Project Paper] (Submitted)
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Abstract
N, N-Dimethylglycine (DMG) is a tertiary amino acid that is commonly used as a nutraceutical in veterinary medicine. It is claimed to have immunomodulating properties such as supporting the production of antibodies and lymphocytes. Notably, recent studies on this topic in cats are lacking, with the last study dating back to 1992. This novel study investigated the effect of DMG supplementation on feline haematological parameters and the percentage of CD4+ and CD8+ T cells after vaccination with single dose of Purevax®Feline 4 core vaccine containing modified live rhinotracheitis-calici-panleukopenia virus and Chlamydia psittaci. Twelve neutered cats from an animal shelter of both genders were divided into control (n=6) and treatment groups (n=6), and were given one dose of vaccination at day 0, with the latter receiving oral DMG supplementation (125mg/mL) at a dosage of 0.5 mL twice daily for 14 days alongside vaccination. Blood samples were collected on day 0 (pre-treatment) and day 14 (post-treatment), and haematological analysis was performed to evaluate parameters such as complete blood count and differential leukocyte count. Peripheral blood mononuclear cells were isolated and subjected to flow cytometry to determine CD4+ and CD8+ T cell percentages. Statistical analyses were conducted using non-parametric T test to identify any significant differences. Haematological analysis revealed that red blood cell, haematocrit, haemoglobin, platelet, white blood cell counts as well as monocyte, eosinophil, basophil percentages showed no significant difference (P>0.05) between control and treatment group after 14 days of experiment. Interestingly, on post treatment (day 14), neutrophil percentage of the treatment group was significantly lower (P=0.0238) compared to the control group while lymphocyte percentage of the treatment group was significantly higher (P=0.013) compared to the control group. As for T cell subset percentages, there was a significant increase (P=0.0022) in CD4+ percentages in the control group from pre-treatment to post-treatment while this was not seen in the treatment group. Both groups exhibited a decrease in CD8+ percentage from pre-treatment to post treatment, however, it was not statistically significant. Lastly, CD4:CD8 ratios of both groups showed no significant difference when comparing pre-treatment values to post-treatment values. In conclusion, DMG supplementation may have a significant effect in decreasing neutrophil percentage and increasing lymphocyte percentage. In contrast, DMG supplementation appears to have no significant impact in influencing T cell subsets percentages particularly CD4+ and CD8+. These results emphasize the need for more research to better understand DMG's effects on feline health and immunity.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms. Nordeena Abdul Aziz |
| Date Deposited: | 18 Nov 2024 02:46 |
| Last Modified: | 18 Nov 2024 02:46 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2139 |
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