Ismail, Nur Amirah (2023) Occurrences and antimicrobial resistance profile of Enterococcus spp. isolated from broilers in Selangor. [Project Paper] (Submitted)
![[img]](http://psaspb.upm.edu.my/style/images/fileicons/text.png) |
Text
lp FPV 2023 100.pdf Download (2MB) |
Abstract
Enterococcus spp. is a gram-positive, facultative anaerobic bacteria that is one of the main microflora of the gastrointestinal tract in birds and mammals. Enterococci is an opportunistic pathogen and has recently become an emerging health issue in the poultry industry. Another rising concern is the public health risk for zoonotic transmission as well as antimicrobial resistance (AMR) enterococci via poultry meat consumption. The aim of this study is to determine the occurrence of Enterococcus spp. from broilers and to examine the antimicrobial resistance profile of the isolated Enterococcus spp. in Selangor. A total of 30 cloacal swabs were collected from broilers from a poultry farm in Selangor. All samples were processed by plating onto selective media to isolate presumptive enterococci. Further identification was carried out using Gram staining, catalase test, and a series of other biochemical tests such as bile esculin test, soluble hemolysin, 6.5% NaCl, arabinose, mannitol, lactose, and additional sugar tests for species identification. Next, the Kirby-Bauer disc diffusion method was used to determine the antimicrobial susceptibility profile of the isolated strains. Overall, the occurrence of Enterococcus spp. was 36.7% of total birds . From 25 pure cultures obtained, 33.3% of isolates were identified as Enterococcus faecalis (E. faecalis), followed by 20% of E. gallinarum, 13.3% of E. faecium. 13.3% were E. cecorum. Enterococcus cecorum has recently been identified as the newly pathogenic strain that affects the musculoskeletal system of birds. Other isolates obtained were classified under Enterococcus spp (others). In the antimicrobial susceptibility testing (AST), 100% of the isolates were found to be resistant to erythromycin (5μg) and tetracycline (30μg). Sixty percent (60%) resistance was found against enrofloxacin (5μg), and 27% were resistant to vancomycin (5μg) . Meanwhile, the majority of the isolates were susceptible to amoxicillin (10μg) at 87%. In conclusion, there are high occurrences of Enterococcus spp. isolated from broilers which constitutes 60% of the total isolates. The results show that poultry is a potential source of multiple-drug resistant enterococci and presents a risk of AMR enterococci in public health. Further investigation should be done to investigate predisposing factors leading to enterococci-associated diseases (EAD) in the poultry industry and strategies to minimize AMR by ensuring the efficiency of antibiotic usage.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms. Nordeena Abdul Aziz |
| Date Deposited: | 18 Nov 2024 02:57 |
| Last Modified: | 18 Nov 2024 02:57 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2141 |
Actions (login required)
 |
View Item |