Mohd. Fikri, Muhammad Faiz (2023) Occurrence of endoparasitic infection and histopathological changes in spotted green pufferfish, Dichotomyctere nigroviridis (Marion de Procé, 1822) in Bagan Lalang, Selangor. [Project Paper] (Submitted)
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Abstract
Dichotomyctere nigroviridis (Marion de Procé, 1822) or also known as spotted green pufferfish belongs to the family tetraodontinae. In Malaysia, the Dichotomyctere nigroviridis can be found widely in fresh and brackish waters and one example is the. Due to its preference for eating crustaceans and molluscs that usually play a role as intermediate hosts of certain parasites, the fish may become infected with parasites, which could pose a risk to the public's health. Since the endoparasite infection of this pufferfish has not yet been documented in Malaysia, the present study aims to evaluate the occurrence of endoparasite infection and histopathological changes in juvenile and adult spotted green pufferfish. This study was carried out in September 2023 in Bagan Lalang, Tanjong Sepat, Selangor. Sixteen fish consisting of 8 juveniles and 8 adults were sampled for endoparasites during necropsy. Numerous amounts of parasites were observed from the kidney consisting of myxozoans, which had a prevalence of 100% in both adults and juveniles. No parasites were observed from the brain, liver, gastrointestinal tract, spleen, heart, muscle and gonad. The Fisher’s Exact Test performed using SPSS v27 statistical software revealed a significant difference (p>0.05) between the adult and juvenile spotted green pufferfish in terms of endoparasite burden. The histological scoring system identified structural changes in the kidney, including the presence of the interstitial pigment (melanomacrophages) and tubular epithelial degeneration or necrosis. The histopathological assessment of various internal organs involved evaluating lesions based on specific criteria, including congestion, the presence of interstitial pigment melanomacrophage (MMC), and lymphocyte infiltration. In brain histopathology, moderate congestion was observed in 60% of adults but none in juveniles. Kidney histopathology revealed severe MMC deposition in 75% of all individuals. Intestinal histopathology showed 33.33% of adults with moderate lymphocyte infiltration, while none was observed in juveniles. In the spleen, 75% of adults exhibited severe MMC deposition, with none observed in juveniles. Heart histopathology revealed cellular degeneration in 37.5% of all individuals, and in the gonad, 50% of all individuals displayed mild congestion. For the PCR sequencing results presence of endoparasite Triactinomyxon type sp. and Ortholinea auratae myxozoan in the green spotted pufferfish was detected. This study's findings contribute to the establishment of a preliminary parasite inventory in the region and lay the foundation for future investigations into the potential consequences of consuming this species.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms Nurhaznita Mahmood |
| Date Deposited: | 19 Nov 2024 03:14 |
| Last Modified: | 19 Nov 2024 03:14 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2170 |
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