Anaesthetic effects of sodium bicarbonate on red tilapia juvenile fish, Oreochromis spp.

Abu Hamid, Muhammad Hanif (2023) Anaesthetic effects of sodium bicarbonate on red tilapia juvenile fish, Oreochromis spp. [Project Paper] (Submitted)

Text lp fpv 2023 45.pdf Download (1MB)

Abstract

Red tilapia fish (Oreochromis spp.) is one of the major species in the aquaculture industry in Malaysia. The welfare of these fish during various procedures is paramount, necessitating the use of suitable anaesthetic agents to minimise stress. This study delves into the anaesthetic potential of sodium bicarbonate, evaluating its effectiveness and safety in red tilapia juvenile fish. A total of 40 fish were randomly assigned to the three concentrations of 25 g/L, 50 g/L, and 75 g/L sodium bicarbonate and 150 ppm tricaine methanesulfonate (MS-222), with n = 10 per treatment group. Two transparent 1-litre tanks were used as the anaesthetic induction and recovery chamber respectively. Fish were transferred into recovery chamber once attaining surgical (Stage IV) anaesthesia, or 15 minutes of immersion, whichever earlier. Fish subjected to sodium bicarbonate at concentrations of 25 g/L and 50 g/L achieved only light anaesthesia (Stage III) within the 15 minutes of immersion. In contrast, fish in 75 g/L and MS-222 reached surgical anaesthesia (Stage IV) at 195 ± 88 and 418 ± 74 seconds, respectively. All 40 fish regained normal swimming patterns within 10 minutes of recovery. However, all fish exposed to sodium bicarbonate died within 2 days post-anaesthesia while all fish in MS-222 survived. In summary, this study shows that sodium bicarbonate can induce light to surgical anaesthesia. However, the 100% mortality post-anaesthesia raises safety concern on its use in red tilapia juvenile fish.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	20 Nov 2024 07:37
Last Modified:	20 Nov 2024 07:37
URI:	http://psaspb.upm.edu.my/id/eprint/2174

Actions (login required)

View Item