Leong, Yuan Hang (2023) Effects of trash fish and pelleted feed on growth, microbiota, and intestinal histology of the juvenile australian red claw crayfish, cherax quadricarinatus (von martens, 1868). [Project Paper] (Submitted)
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Abstract
In the past decade, Australian red claw crayfish (Cherax quadricarinatus) has gained attention as a promising species in local aquaculture due to its rapid growth and exceptional adaptability, making it a focal point of aquaculture research. Similar to the trend with other aquaculture species, feed input is a critical factor affecting crayfish farming. In local crayfish cultivation, the feeding regime incorporates a commercially formulated diet, pelleted feed, and traditional sources like trash fish. However, the use of trash fish is controversial due to its impact on farming sustainability and potential health issues. Therefore, the study was to investigate the effects of trash fish and pelleted feed on growth performance, gut microbiome analysis, and the intestinal histology of juvenile crayfish. A total of 40 crayfish were acclimatised for one week and cultured for four weeks in two groups (group A and group B). Group A served as the control group, fed with TIGER prawn feed size (TT663) as pellet, and Group B acted as the treatment group, fed with rainbow sardine (Dussumieria acuta) as trash fish. Growth performance indices were recorded and calculated. The survival rate in Group A was significantly higher (p < 0.05) than those in Group B. However, there were no significant differences (p > 0.05) in other growth performance indices between the two groups. High-throughput sequencing techniques were employed to analyse the alterations in intestinal microbiota composition. The dominant phyla in the intestines of both groups were Proteobacteria, and Firmicutes. The species richness and diversity of the microbiota in Group A were higher than in Group B. Intestinal histology was evaluated using Image Analyser and ImageJ software. Intestinal histological indices, including villus height, villus height to crypt depth ratio, villus surface area, lamina propria thickness, and goblet cells number in Group A, were significantly different (p < 0.05) than in Group B. In conclusion, pellet-fed crayfish had a significantly higher survivability, improved intestinal health and substantial microbial population compared to trash fish-fed crayfish. These findings underscore the significance of tailored nutrition in aquaculture, advocating for the adoption of formulated diets like pelleted feed to optimise crayfish production.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Veterinary Medicine |
| Depositing User: | Ms Nurhaznita Mahmood |
| Date Deposited: | 20 Nov 2024 07:49 |
| Last Modified: | 20 Nov 2024 07:49 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2178 |
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