Preparation and characterization of tilapia fish scale gelatin base film.

Mohd Asshazali, Muhammad Affifuddin (2018) Preparation and characterization of tilapia fish scale gelatin base film. [Project Paper] (Submitted)

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Abstract

The economy of freshwater fish in Malaysia reported to be increasing in aquaculture production. It is about 33% of the fresh water fish are contributed by tilapia. The increase rate of tilapia production, causing by the high demand of tilapia fish, thus contribute to the by-product of the fish. This amount of waste from Tilapia is a useful resource for collagen extraction. By, optimizing yield based on the extraction method and heating temperature time of fish gelatin extraction. Also, determine whether effects of different extraction temperature and time will effect that mechanical properties of the film. This research was carried out to observe the changes in the properties of Tilapia fish scale films by comparing film at different temperature and extraction time. Furthermore, Glycerol was added to the film as a plasticizer. The film thickness film corporate with glycerol is between 0.14 mm to 0.16 mm and film that purely without addition of glycerol is between 0.10 mm to 0.12 mm. While the tensile strength and elongation at break were studied to determine the mechanical properties of the film. The scanning electron microscopy (SEM) was done to study the morphology of surface film between fish scale gelatin film corporate with glycerol and the pure fish scale gelatin film. While for FTIR the result shown that the amide band A, B, I, II and III were found in both films with or without glycerol. The IR ratios (intensity ratio) of all the collagen extracted from tilapia fish scale were 1.17 for both film (with glycerol and without glycerol). The intensity ration is used to indicate the triple helical structure of collagen. All of these results prove that the helical structure of the collagen were reserved properly in good conditions. Next, the current study found that the colour do changes from transparency white to yellowish milky white when the extraction method at the highest temperature and longer time. The result shows that, as heating temperature and thermal treatment time was increased the tensile strength of films increase. Besides, Fourier transform infrared spectroscopy (FTIR) proved that the collagen of these fish were integrated and native. These experiments confirmed that the addition of glycerol as plasticizer influences the mechanical, physical and the amino acid content in the film. In conclusion, these findings suggested that fish waste skin, scale, bone and fin collagen possess the potential to be an alternative collagen source for a variety of uses in many fields.

Item Type:	Project Paper
Faculty:	Faculty of Engineering
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	09 Oct 2025 07:10
Last Modified:	09 Oct 2025 07:10
URI:	http://psaspb.upm.edu.my/id/eprint/2188

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