Cloning the PvuII isoschizomer restriction modification system from JMH 1 (4) SM clone into Escherichia coli

Lee, Swee Ling (2007) Cloning the PvuII isoschizomer restriction modification system from JMH 1 (4) SM clone into Escherichia coli. [Project Paper] (Submitted)

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Abstract

Cloning the Pvull isoschizomer restriction modification system from JMH 1 (4) SM into Escherichia coli was studied. The presence of the isoschizomer for Pvull in bacteria strain JMH 1(4) SM was verified. Selected commercially available restriction enzymes were used to construct restriction pattern of bacteria strain JMH 1(4) SM. DNA fragments of2-5 kb DNA were obtained by digesting genomic DNA of bacteria strain JMH l (4) SM by using selected restriction enzymes Eco RI, HindIII and FauNDI. The DNA fragments were ligated into the linear plasmid pET23a (+) and transformed into TOPl0F' Escherichia coli. The recombinant plasmids were extracted and digested with lysate of bacteria strain of JMH 1 (4) SM to verify the presence of methylase gene. However, the presence of methylase activity was not confirmed by further transformations.

Item Type:	Project Paper
Faculty:	Faculty of Agriculture and Food Sciences
Depositing User:	Mr. Azman Mohamad
Date Deposited:	17 Dec 2024 02:43
Last Modified:	17 Dec 2024 02:43
URI:	http://psaspb.upm.edu.my/id/eprint/2277

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