Aldi Fasri, Alliffi Erijuffaldi (2010) Anti-inflammatory effects of the ethanolic extract of Acanthopanax trifoliatus (L) Merr leaves in acute and chronic animal models of inflammation. [Project Paper] (Submitted)
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Abstract
Summary: The leaves of Acanthopanax trifoliatus (L) Merr have been used to treat several diseases such as tuberculosis and lung hemorrhage, and as a tonic to improve general weakness. This study was conducted to determine the effect of ethanolic extract of Acanthopanax trifoliatus. (L) Merr on both acute and chronic anti-inflammatory activity. The male Sprague-Dawley rats were randomly divided into five groups, with 6 animals per group for both acute and chronic inflammation. For inflammation study: (1) control group (5% ethanol); (2) group treated with 30 mg/kg of extract; (3) group treated with 100 mg/kg of extract; (4) group treated with 300 mg/kg of extract; (5) reference group 30mg/kg of piroxicam for acute inflammation and 10 mg/kg of indomethacin for chronic inflammation. For acute inflammation study, the rats were injected subcutaneously with 0.1 ml of 1% carrageenan onto the plantar surface of right hind paw and equal volume of distilled water was injected onto the plantar surface of the left hind paw after 30 minute force fed of ethanolic extra of Acanthopanax trifolitus (L) Merr leaves (EAT).The volume of both hind of each rat was measured using a plethysmometer at every half- hourly interval until the period of five hours after the injection of the carrageenan. For chronic inflammation study, the rats were injected intradermally with 0.1ml of Freund’s Complete Adjuvant (FCA) onto the right hind paw. The rats were acclimatized for 14 days after the adjuvant injection. The treatment was started on day 14 until day 28. The volume of the paw was measured before induction, before treatment, and after treatment using plethysmometer. The percentage of swelling and inhibition for all group in both acute and chronic inflammation was calculated and compared. For acute inflammation, EAT gave significant anti-inflammatory effect at EAT 300 mg/kg and piroxicam 30 mg/kg as compared to control group and the percentage of inhibition for the treated group of 30, 100 and 300 mg/kg were 10.50%, 32.43% and 46.23% respectively. For chronic inflammation, EAT exhibited significant effect also at 300 mg/kg and indomethacin 10 mg/kg as compared to control group and the percentage of inhibition for treatment group of 30, 100, 300 mg/kg were 9.57%, 23.71% and 47.57% respectively. It can be concluded that EAT exhibited a dose dependent inhibitory effect towards oedema in both acute and chronic infalammation. These result has shown that EAT leaves have anti-inflammatory properties. Further studies should be done to obtain effective dose (ED50) and may increase the dosage for EAT leaves treatment. Toxicity study (LD50) also should be carried out in order to determine the health safety usage in high dose concentration.
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms Norafizah Radzuan |
| Date Deposited: | 22 Nov 2022 07:36 |
| Last Modified: | 15 Feb 2023 01:30 |
| URI: | http://psaspb.upm.edu.my/id/eprint/237 |
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