Yusof, Aina Nadirah (2019) Association between urban traffic air pollution and DNA damage in buccal mucosa cells of children in primary schools in Klang Valley. [Project Paper] (Submitted)
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Abstract
Introduction: Traffic-related air pollution (TRAP) that are currently increasing due to increment in numbers of vehicles possessed genotoxic properties that are potential in inducing genetic damage. Micronuclei (MN) frequency is used as an indicator for identifying potential genotoxic exposures. Objective: This study aims to determine the association between TRAP and frequency of MN in buccal mucosa cells of children and reported respiratory health symptoms as well as determining the main factor influencing them. Methodology: A comparative cross-sectional study was carried out among primary school children in the urban area with high density of traffic (N=94, Kuala Lumpur) and sub-urban area with less density of traffic {N=94, Hulu Langat) through stratified sampling for healthy children without respiratory illness history as well as free from exposure of radiation who fulfilled inclusive criteria. A questionnaire adapted from American Thoracic Society and International Study of Asthma and Allergies in Childhood (ISAAC) was distributed to obtain reported respiratory symptoms. Exfoliated buccal mucosa cells sample were collected and analyzed to determine the frequency of micronuclei. Air quality assessment was also carried out in a total of 6 schools ( exposed and comparative) by using TSI DustTrak DRX Aerosol Monitor 8534 for measurement of PM2.5 and PM10, LaMotte's Model BD Air Sampling Pump for measurement of NO2 and SO2, and TSI Q-TRAK 7565 for measurement of CO, CO2. Buccal mucosa cell sample collected were analysed in laboratory whereas questionnaire and air monitoring data collected were analysed by using Statistical Package for Social Science (SPSS) v.22. Results: The concentration of TRAP at exposed schools were significantly higher compared to comparative schools; PM10 (p-value = 0.046; t-value = -1.993), PM2.5 (p-value = 0.046; t-value = -1.993), NO2 (p-value = 0.037; t-value = -2.087), SO2 (p-value = 0.003; t-value = -6.709), CO (p-value = 0.037; t-value = -2.087) ,TVOC (p-value = 0.037; t-value = -2.087). TRAP (PM10, PM2.5, NO2, SO2, CO, TVOC) were found significantly associated with respiratory symptom phlegm (p <0.001). TRAP (PM10, PM2.5, NO2, SO2, CO, TVOC) were also found significantly associated with MN frequency (p<0.001 ). Additionally, the micronuclei (MN) frequency in exposed group was significantly higher (18.5(5.5)] than in comparative group (0.50 (1.0)], and the difference was significant (Z= -11.946, p<0.001 ). Conclusion: This study concludes that exposure to TRAP increase the risk of respiratory symptoms and might have an impact on MN formation among children. Therefore, this study serves as baseline data for genotoxic damage among children in vicinity of traffic in urban area specifically. Keywords: TRAP, Children Respiratory Health, Micronuclei, DNA Damage
| Item Type: | Project Paper |
|---|---|
| Faculty: | Faculty of Medicine and Health Science |
| Depositing User: | Ms Norafizah Radzuan |
| Date Deposited: | 27 May 2025 08:12 |
| Last Modified: | 27 May 2025 08:12 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2414 |
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