Mutagenicity assessment of paddy water from Tanjung Karang, Selangor using Salmonella typhimurium TA100 strain: a preliminary study

Rohaimi, Nurul Aishah (2019) Mutagenicity assessment of paddy water from Tanjung Karang, Selangor using Salmonella typhimurium TA100 strain: a preliminary study. [Project Paper] (Submitted)

Text lp FPSK4 2019 45 REPAIR 2.pdf Download (11MB)

Abstract

Introduction: An increasing number of industrial, agricultural and commercial chemicals in the aquatic environment lead to various deleterious effects on organisms. Among different agricultural activities, paddy crops plantation become concern as paddy water might be polluted due to anthropogenic activities and also usage of pesticides by farmers. Objective: This study was conducted to determine the mutagenic activity of paddy water from Tanjung Karang, Selangor using Salmonella typhimurium TA 100 strain. Methodology: The sampling was done at paddy field of Kampung Sawah Sempadan, Tanjung Karang, Selangor where paddy water samples were collected from 9 different points and extracted by using Solid Phase Extraction (SPE). The Ames mutagenicity assay using genetically mutated strain of Salmonella typhimurium (TA l00) without metabolic activation (S9 mixture) was used to determine the mutagenic activity of the paddy water samples. In the meantime, several physicochemical properties of water samples were measured. Results: Based on the first time point ( early plantation), Inlet 2, Point 1, Point 2, Outlet 2, and Outlet 3 were shown to be positive to induce mutagenic activity with the 153 ± 129 to 847 ± 804 of total mean of revertants colonies. Based on the second time point (preharvesting), it was showed that Inlet 3, Point 1, Point 2, and Point 3 were positive to induce mutagenic activity with 175 ± 147 to 281 ± 248 of total mean number of revertants. However, there was no statistically significant difference between sampling points and total mean revertants colonies with different sampling times (p>0.05). Conclusion: In summary, some of the paddy water samples showed mutagenicity effects in TA 100 strain. Therefore, it is important to screen the mutagenic activity of environmental samples as the event is irreversible and may • eventually lead to cancer formation. Keywords: Paddy water, Mutagenicity, Ames Test

Item Type:	Project Paper
Subjects:	R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	25 Feb 2026 06:01
Last Modified:	25 Feb 2026 06:01
URI:	http://psaspb.upm.edu.my/id/eprint/2735

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