Optimization of erythritol production using Yarrowia lipolytica and pure glucose as a substrate

Saharuddin, Anis Athirah (2022) Optimization of erythritol production using Yarrowia lipolytica and pure glucose as a substrate. [Project Paper] (Submitted)

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Abstract

Erythritol is a natural sweetener used in many products, due to its natural properties, especially in food, since it has almost zero-calorie content and zero Glycemic Index (Regnat et al., 2018), making it a practical and safe ingredient for people with diabetes and obesity. According to the National Health and Morbidi1y Survey in 2019, Malaysian adults have been living with diabetes, including children, due to excessive sugar and fat consumption. Therefore, erythritol has been introduced due to its advantages for diabetes and obese patients. However, erythritol production has not been widely used and established, making it expensive due to low production and fewer demands. The current study shows that only 200 g/L of erythritol yield has been produced in 7 days of fermentation with pH and stirring as the factors that will affect the erythritol production by using yeast Yarrowia lipolytica and glycerol as a substrate. The objectives were to study the effect of the stirring speed, airflow rate, and pH on the dry cell weight of yeast Y. lipolytica, optical density (OD600), glucose concentration, erythritol concentration, and optimize the processing parameters. This study produced erythritol using the yeast Yarrowia Lipolytica and pure glucose as a substrate. Various factors influenced the erythritol production, including stirring speed in the range of200 rpm- 300 rpm, airflow rate at 1.0 L/min -2.0 L/min, and pH at 5.0 - 6.0. The factors were studied using response surface methodology (RSM) software to obtain the optimum conditions. Dry cell weight of yeast, OD600, glucose concentration, and erythritol concentration have been analyzed. Based on the results, it was found that pH and stirring significantly affect the dry cell weight. Meanwhile, stirring and airflow rate significantly affects the erythritol concentration. pH and stirring also slightly change the OD6oo and glucose concentration. The correlation analysis was done to determine the linear relationship between the responses. OD600 and dry cell weight results in a moderate negative relationship; as dry cell weight increases, the OD600 rises. Meanwhile, a strong negative relationship correlation was shown for glucose and erythritol concentration, in which erythritol concentration rises when glucose concentration falls. Therefore, dry cell weight and erythritol concentration were chosen to optimize the responses as these two responses are more accurate than the others. Moreover, the combined factors show that they significantly affect the responses. Therefore, as optimization was carried out, it was found that the dry cell weight of the yeast and concentration of erythritol would give the highest amount, which is 23.9279 g/L and 204.458 g/L when operated at a stirring speed: 150 rpm, airflow rate: 1.50 L/min and pH: 5 .52. After validation of dry cell weight and erythritol concentration was carried out, it was found that the percentage error for both responses was not above 15%. Therefore, the optimum condition is suitable for producing erythritol.

Item Type:	Project Paper
Subjects:	T Technology > TS Manufactures
Faculty:	Faculty of Engineering
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	09 Apr 2026 03:35
Last Modified:	09 Apr 2026 03:35
URI:	http://psaspb.upm.edu.my/id/eprint/2763

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