Pathogenicity of fowl adenovirus isolates of Malaysia in specific pathogen free embryonated chicken eggs

Mustafa Kamal, Nurul Kamaliah (2015) Pathogenicity of fowl adenovirus isolates of Malaysia in specific pathogen free embryonated chicken eggs. [Project Paper] (Submitted)

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Abstract

Fowl Adenovirus (FAdV) is known to be the primary pathogen of inclusion body hepatitis (IBH) and can cause high mortality in susceptible chickens. It was the objective of this study to determine the pathogenicity of recent FAdV isolates of Malaysia in specific pathogen free (SPF) embryonated chicken eggs. Thirty nine 8-day-old SPF embryonated chicken eggs were divided into three groups namely groups A, B and C. Twelve eggs from groups A and B were inoculated with 0.1mL/egg of FAdV isolates A (UPM11134) and B (UPM1127), respectively via chorioallantoic membrane (CAM) route. Fifteen eggs from Group C were left uninoculated and used as the control group. Three eggs from the control group were sacrificed prior to FAdV inoculation. All eggs were candled twice daily and any mortality was recorded throughout 14 days trial. Samples of liver, gizzard and CAM of the dead embryos were fixed in 10% buffered formalin for histological examination. The study showed that both FAdV isolates caused 100% mortality when inoculated in 8-day-old SPF eggs within 3 to 8 days post inoculation (pi). The control group remained no mortality throughout the trial. The gross lesions recorded were mainly in CAM, liver and gizzard. The CAM was thickened and cloudy as early as day 3 pi. Lesions in liver revealed enlarged and ecchymotic haemorrhage was first observed at day 3 pi. The lesion became severe with the presence of hydropericardium and multifocal areas of necrosis in liver on day 7 pi. The gizzard was normal for both groups. Histologically, basophilic intranuclear inclusion bodies were observed in CAM, liver and gizzard in groups A and B. The control group remained normal throughout the trial. It was concluded that the FAdV isolates of Malaysia is highly pathogenic to SPF embryonated chicken eggs and the embryonic liver should be used for isolation and propagation of the virus.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	08 Nov 2022 02:25
Last Modified:	08 Nov 2022 02:25
URI:	http://psaspb.upm.edu.my/id/eprint/318

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