Detection of Orf virus using 4 different set of primer from clinical cases of Orf in goat’s from Program Ladang Angkat UPM

Ismail, Muhammad Farid (2015) Detection of Orf virus using 4 different set of primer from clinical cases of Orf in goat’s from Program Ladang Angkat UPM. [Project Paper] (Submitted)

Text fpv 2015 37.pdf Download (2MB)

Abstract

Contagious ecthyma is an acute, contagious, debilitating and economically important viral skin disease of sheep, goat and some other domesticated and wild ruminants. The etiological agent for this disease is Orf virus where the virus is under the genus Parapoxvirus and family Poxviridae. Two farms from programme Ladang Angkat had outbreak with contagious ecthyma. Three sample were obtained from the outbreak. The scab samples were collected from animals were homogenized and the DNA was extracted manually. The targeted DNA were then amplified by using Polymerase Chain Reaction (PCR). 4 set of primers were used in this study in both interferon resistant (VIR) gene of the virus (VIRF-VIRR; ORFVO59F-ORFV059R) and the major envelope proteins (PPP1-PPP4, semi nested PPP3-PPP4, ORFVO11F-ORFVO11R). Phylogenetic trees were built by comparison of the complete coding area for both 011 and 059 genes. The result of this study showed that there is no significant difference between all the primers used in this study. Both samples 2 and 3 were so closed phylogenetically to each other compared to sample 1 according B2L whole coding gene. The Orf virus based on B2L gene isolated from this cases are different from the virus strain from east south Asia strains. For Orf virus based on 059 gene from this case are different to each other but closely related to some Chinese Orf virus strain.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	16 Nov 2022 02:10
Last Modified:	16 Nov 2022 02:10
URI:	http://psaspb.upm.edu.my/id/eprint/339

Actions (login required)

View Item