Determination of E. Coli contamination on keyboard and mouse among university student

Mohd Dzukri, Syamim Athira (2016) Determination of E. Coli contamination on keyboard and mouse among university student. [Project Paper] (Submitted)

Text lp FPSK4 2016 13.pdf Download (14MB)

Abstract

Introduction: Computer has become as one of the important tools in higher education. Computers are widely used in computer laboratory, printing shops and library to access to internet to obtain information resources. Most of these locations are generated for multiple user computers. As the population of such facility increases, the computer acts as a reservoir for the transmission of potential pathogenic bacteria such as E. coli. Objective: The aim of this study is to determine and compare E. coli contamination in keyboard and mouse at different locations (printing shops, computer laboratory and library). Methodology: Swab samples were collected by using sterile swab at three different places which is at printing shop, library and computer laboratory. Then, the samples were put into the lauryl sulphate broth and were incubated overnight at 37°C. The Mac Conkey agar is used as selective agar to determine the presence of E. coli in keyboard and mouse. Results: The E. coli colonies were detected on keyboard and mouse at printing shop, library and computer laboratory. Both keyboard and mouse has highest E. coli count with 98.0 cfu/mL and were found at library and printing shop respectively. The lowest E. coli count is 0.0 cfu/mL for keyboard and mouse and it found at all three locations (printing shop, computer laboratory and library). There is significant difference of E. coli contamination between all three places (printing shop, library, computer laboratory). Conclusion: The E. coli contamination on keyboard and mouse are really needed to be alarmed regarding to the awareness on cleaning and disinfecting the keyboard and mouse after the operational time. The contamination of E. coli on keyboard and mouse might be due to the poor personal hygiene of user or its surrounding. Keywords: E. coli, computer; keyboard, mouse, multiple user computer

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Mr Muhamad Fazril Awang
Date Deposited:	23 Nov 2022 03:24
Last Modified:	23 Nov 2022 03:24
URI:	http://psaspb.upm.edu.my/id/eprint/376

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