Effects of ascorbic acid (vitamin C) on growth performance and susceptibility to Aeromonas hydrophila infection in juvenile African catfish, Clarias gariepinus

Latif, Siti Nadhirah (2015) Effects of ascorbic acid (vitamin C) on growth performance and susceptibility to Aeromonas hydrophila infection in juvenile African catfish, Clarias gariepinus. [Project Paper] (Submitted)

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Abstract

African catfish, Clarias gariepinus, is one of the most popular freshwater food fish. Due to high demand for human consumption, the fish is intensively reared in Malaysia. Ascorbic acid (vitamin C) is an essential dietary supplement for normal physiology functions in animals including fish and plays an important role in the health status of fish. However, catfish cannot synthesize vitamin C by itself and the vitamin is easily oxidized during pelleting and storage. Therefore, this experiment is conducted to assess the effectiveness of supplementation of vitamin C on growth performance and to determine the optimum level of vitamin C needed to mitigate Aeromonas hydrophila infection in juvenile African catfish. Eighty juveniles were allocated randomly into eight tanks (10 fishes per tank) i.e. Group A (four tanks) for growth performance study and Group B (four tanks) for challenge study with A. hydrophila. The juveniles were kept at water temperature of 27-28°C, 4 ppm D.O., pH 7-8, and 0.0 ppm ammonia. Initially, body weight of the fishes was weighed to the nearest gram to formulate the supplemented diet. The fish were fed twice daily using commercial diet at 10% of total body weight (BWT) supplemented with vitamin C at 0 mg/kg BWT, 30 mg/kg BWT, 90 mg/kg BWT and 240 mg/kg BWT. On Day-11, the fish in Group B were injected with A. hydrophila intraperitoneally. Then, their body weight and survival rate were noted every five days for 20 days. It was found that the growth rate for Group A inversely decreased with vitamin C concentration. However, no mortality of fish was observed in Group A. The growth rate for Group B was similar to Group A but the survival rate of fish was increased as the dosage of vitamin C was increased. Tissue samples (brain, liver, intestine, spleen, kidney) were taken from three survived fish from each tank for microscopic examination.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	21 Nov 2022 06:39
Last Modified:	21 Nov 2022 06:39
URI:	http://psaspb.upm.edu.my/id/eprint/404

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