Isolation and identification of bacteria in the respiratory tract of the Red Jungle Fowl

Omar, Fatin (2016) Isolation and identification of bacteria in the respiratory tract of the Red Jungle Fowl. [Project Paper] (Submitted)

Text fpv 2016 15.pdf Download (1MB)

Abstract

Respiratory diseases are the most important diseases affecting poultry and this has been observed in domesticated Red Jungle Fowl. The objectives of this present study were to isolate and identify bacteria from sinus and lung of Red Jungle Fowl and to determine the relationship between bacteria isolated with respiratory tract problem. Sixteen Red Jungle Fowl consisting of 12 clinically unhealthy and four apparently healthy were sampled from privet farm. The chickens were slaughter and then sinus swabs and lung samples were collected from each chicken. All samples were cultured on the blood and chocolate agar for bacteria isolation. The isolated bacteria was then were identified using biochemical tests. Nineteen Gram positive (43.2%) and 25 Gram negative (56.8%) bacteria were isolated from clinically unhealthy Red Jungle Fowl. Five Gram positive (41.7%) and seven Gram negative (58.3%) bacteria were isolated from apparently healthy Red Jungle Fowl. There was no significant difference between clinically unhealthy and apparently healthy Red Jungle Fowls in term of frequency of Gram positive and Gram negative bacteria isolated (p > 0.05). The bacteria species isolated from clinically unhealthy chickens were Staphylococcus species (25%), Corynebacterium species (18.20%), Avibacterium avium (9.09%), Neisseria species (6.82%), Gallibacterium anatis bv. Haemolyticatis (6.82%), Escherichia coli (6.82%), Avibacterium gallinarum (6.82%), Proteus mirabilis (4.55%), Pasteurella multocida subspecies multocida (4.55%), Pasteurella sp/A (4.55%), Streptococcus species (2.27%), Pseudomonas aeruginosa (2.27%), and Aeromonas sp (2.27%). The bacteria species isolated from apparently healthy chickens were Staphylococcus sp (16.67%), Corynebacterium species (16.67%), Avibacterium avium (16.67%), Bacillus species (8.33%), Gallibacterium anatis bv haemolyticatis (8.33%), Escherichia coli (8.33%), Pseudomonas aeruginosa (8.33%), Pasteurella multocida subspecies multocida (8.33%) and Pasteurella haemolytica (8.33%). It can be concluded that the domesticated Red Jungle Fowl harbour numerous opportunistic bacteria that might contribute to the respiratory problem in immunocompromised chickens.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Siti Mariam Giman
Date Deposited:	22 Nov 2022 07:18
Last Modified:	22 Nov 2022 07:18
URI:	http://psaspb.upm.edu.my/id/eprint/429

Actions (login required)

View Item