Alterations in glutathlone s-transferase and acetylcholinesterase activities in african catfish (clarias gariepinus) following chlorpyrifos exposures

Ahmad, Mohammad Nazrin (2016) Alterations in glutathlone s-transferase and acetylcholinesterase activities in african catfish (clarias gariepinus) following chlorpyrifos exposures. [Project Paper] (Submitted)

Text lp FPSK4 2016 15 .pdf Download (13MB)

Abstract

Introduction:Chlorpyrifos (CPF) is commonly used for pest and insect control in agricultural fields and surrounding freshwater reservoirs. Glutahthione S-transferase (GST) and acetylcholinesterase (AChE) are fish biomarkers used to measures the cellular, biochemical, molecular, or physiological change in an organism that indicate exposure to or the effects of environmental contaminants. Objective: To evaluate the effects of CPF posed to the human by using fish as a vertebrate model. Method: In this study, Clarias gariepinus were artificially propagated. Thereafter, groups of juvenile C. gariepinus been exposed to three graded of chlorpyrifos at concentration (50µg/L, 100µg/L, 150µg/L) for 21 days. Meanwhile, in this research the non-exposed groups was considered as the control group. GST activity was measured in the liver and AChEactivity was measured in the brain using enzymelinked immunesorbent assay method (ELISA). Result and discussion: The finding showed that there were significant differences between the control groups with the exposed groups for AChE activities in the brain and GST activities in the liver after exposure for 21 days. Conclusion:These results show that CPF may cause direct cellular injury in the brain and the liver, thus suggest that AChE and GST may be used as a bio indicators to raise awareness about the potential adverse impacts of CPF on human health. Keywords: Biomarkers, Acetylcholinesterase, Glutathione S-transferase, Chlorpyrifos

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Mr Muhamad Fazril Awang
Date Deposited:	23 Nov 2022 03:34
Last Modified:	23 Nov 2022 03:34
URI:	http://psaspb.upm.edu.my/id/eprint/434

Actions (login required)

View Item