Genetic polymorphism of T344C of CYP11B2 gene in Malaysian and stage renal disease (esrd) subjects

Zulkifli, Nurul Fasihah (2011) Genetic polymorphism of T344C of CYP11B2 gene in Malaysian and stage renal disease (esrd) subjects. [Project Paper] (Unpublished)

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Abstract

Summary: Polymorphism of CYP11B2 gene at T344C of promoter region of CYP11B2 gene was vastly studied in various populations in relation to end-stage renal disease (ESRD). The objective of this study was to determine the T344C polymorphism of CYP11B2 gene in Malaysian ESRD subjects. This study involving 165 Malaysian ESRD subjects and 165 controls. Genomic DNA was extracted from buccal cells of 165 samples using commercially available kits. For T344C gene of CYP11B2, the 152bp products were amplified by Polymerase Chain Reaction (PCR) followed by Restriction Fragment Length Polymorphism (RFLP) method. The 152bp PCR products were digested with Hea III restriction enzyme for 3 hours at 37ºC. The restricted fragments showed 152 bp, 56bp and 97bp. The fragments produced were separated by metaphor agarose gel electrophoresis. The frequency of the allele was counted based on the band showed by the gel. The restricted fragments of 152bp represent TT allele (wild type), 152bp and 97bp represent the TC allele (heterozygous) and 97bp and 56bp represent CC allele (mutant). The frequency of TT, TC and CC genotypes of T344C in CYP11B2 gene in ESRD subjects were 98(59.39%), 60(36.36%) and 7(4.24%), and 92(55.76%), 70(42.42%) and 3(1.82%) for control subjects, respectively. T344C polymorphism of CYP11B2 gene show no significant when compared to control subjects. The T allele of T344C polymorphism of CYP11B2 gene cannot be considered as a possible genetic marker or predisposing risk factor for ESRD in Malaysian subjects. However, this study has to be further continued with more cases and control subjects to confirm the association of CYP11B2 gene mutation with ESRD.

Item Type:	Project Paper
Faculty:	Faculty of Medicine and Health Science
Depositing User:	Ms Norafizah Radzuan
Date Deposited:	23 Nov 2022 03:45
Last Modified:	15 Feb 2023 00:46
URI:	http://psaspb.upm.edu.my/id/eprint/448

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