Isolation and identification of normal flora from the cloaca of Malayan box turtles

Raidi, Syadatul Akma (2016) Isolation and identification of normal flora from the cloaca of Malayan box turtles. [Project Paper] (Submitted)

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Abstract

Malayan Box turtle, Cuora amboinensis kamaroma is a reptile, of the order Testudines, under the family of Geoemydidae and subfamily Geoemydinae. This species is listed by the International Union for Conservation of Nature and Natural Resources (IUCN) Red List of Threatened Species as a vulnerable species in Malaysia. The aim of this study is to determine the presence of bacteria in the cloaca of Malayan Box Turtle. A total of 8 cloacal samples were collected from adult, healthy Malayan Box turtles by using sterile swabs. Bacteria isolation and identification were done to determine species of the bacteria. Nine species of bacteria were isolated and identified from the samples. Only one species of Gram positive and 8 species of Gram negative bacteria were isolated and identified which include Staphylococcus aureus, Acinetobacter lwoffi, Escherichia coli, Pasteurella testudinis, Pantoea agglomerans, Acinetobacter calcoaticus, Salmonella spp., Klebsiella oxytoca and Serratia sp.. Most of the isolated bacteria were normal flora except for S. aureus, E. coli, Salmonella spp., K. oxytoca and Serratia sp. which are zoonotic. These microorganisms can cause severe infection especially in people who are sick or immunocompromised. In conclusion, appropriate hygienic precautions need to be taken during handling and transporting of the animal to prevent any transmission of zoonotic bacteria from the animal to human.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms Nurhaznita Mahmood
Date Deposited:	19 Dec 2022 02:17
Last Modified:	19 Dec 2022 02:17
URI:	http://psaspb.upm.edu.my/id/eprint/662

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