Investigation of bovine growth hormone (BGH) gene in buffalo bulls (Bubalus bubalis) using PCR method

Thomeeran, Harizwan (2017) Investigation of bovine growth hormone (BGH) gene in buffalo bulls (Bubalus bubalis) using PCR method. [Project Paper] (Submitted)

Text lp FPV 2017 42.pdf Download (1MB)

Abstract

The aim of this study was to investigate the ability of specific primers in detection of the bovine growth hormone (bGH) gene in buffalo bulls (Bubalus bubalis) using PCR. The study was carried out in Crossbred Murrah buffalo and Swamp buffalo bulls maintained at Buffalo Breeding and Research Centre, Department of Veterinary Services and Animal Industry, Telupid, Sabah. Genomic DNA from 12 healthy buffalo bulls was extracted and amplified using primers that were designed from the cattle growth hormone (GH) gene sequences. The DNA quality was checked by running the samples in 2% agarose gel electrophoresis. PCR method was used to amplify the growth hormone fragments. GH1, 428 bp fragments of the growth hormone gene was amplified with forward (5’-CGG ACC GTG TCT ATG AGA AGC TGA AG-3’) and reverse (5’-GTT CTT GAG CAG CGC GTC GTC A-3’) primer sequences. GH2, 211 bp fragments of the growth hormone gene was amplified with forward (5’-GCT GCT CCT GAG GGC CCT TC-3’) and reverse (5’-CAT GAC CCT CAG GTA CGT CTC G-3’) primer sequences. The amplification success for PCR showed negative results for both GH1 and GH2 fragments and the mismatch of the primers could be one of the contributing factors. This study to identify the bovine growth hormone (bGH) gene was unsuccessful.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	25 Jan 2023 05:43
Last Modified:	25 Jan 2023 05:43
URI:	http://psaspb.upm.edu.my/id/eprint/723

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