Pathogenicity and immunogenicity of live attenuated and inactivated fowl adenovirus in commercial broiler chickens

Chong, Zheng Zhe (2017) Pathogenicity and immunogenicity of live attenuated and inactivated fowl adenovirus in commercial broiler chickens. [Project Paper] (Submitted)

Text lp FPV 2017 48.pdf Download (2MB)

Abstract

Fowl adenovirus (FAdV) is a non-enveloped DNA virus which is the primary pathogen of inclusion body hepatitis (IBH) in chickens. IBH outbreak is worldwide distributed and being reported in different states of Malaysia. To control the outbreak in poultry industry, vaccination programmme is crucial. However, there is lack of research regarding development of local vaccine against FAdV infections. The objective of this study was to determine pathogenicity and immunogenicity of live attenuated and inactivated FAdV (UPM 1137) in commercial broiler chickens. Fifty four, 1-day-old Cobb 500 broiler chicks were divided into four groups, namely groups A, B, C and D (control). Feed and water were provided ad-libitum. The chicks in groups A, B and C were inoculated with inactivated FAdV (0.2mL) with virus titre of 106.5 TCID50/0.2mL, live attenuated FAdV (0.1mL) with virus titre of 105.2 TCID50/0.1mL, combination of the inactivated (0.2mL) and live attenuated (0.1mL) FAdV, respectively on day 1 (12 chicks of each group) and day 14 of age (6 chicks of each group). Body weight and blood samples were collected prior to necropsy on days 14 and 28 of age, except sampling was also conducted on day 1 of age in the control group. On necropsy, the gross lesions and liver weight were recorded, and samples of liver were collected for histological examination. The study showed that neither clinical signs nor gross lesions were recorded throughout the trial. The body weight of chicken on days 14 and 28 were not significantly different (p>0.05) among all the groups. The liver to body weight ratio of group C was significantly higher (p<0.05) than groups A and D on day 28. There was no gross and histopathological lesions seen in the liver of all the chicken throughout the trial. The FAdV antibody titre in group D (Control) was 938±1596 on day 1 of age and was not detected on day 14 and day 28 of age. However, the FAdV antibody was induced at high titre in all the inoculated groups at day 14 and day 28 of age. The FAdV antibody titre of group C was significantly (p<0.05) higher than groups B and D on day 28. In conclusion, live attenuated and inactivated FAdV used in the study have a low pathogenicity but able to induce production of antibody among the chickens. The combination of live attenuated and inactivated FAdV has higher immunogenicity compared to sole usage of live attenuated FAdV.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	08 Feb 2023 02:49
Last Modified:	08 Feb 2023 02:49
URI:	http://psaspb.upm.edu.my/id/eprint/729

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