Occurrence of pathogenic bacteria in blood cockles, Anadara granosa

Jantan, Nurin Syakirin (2017) Occurrence of pathogenic bacteria in blood cockles, Anadara granosa. [Project Paper] (Submitted)

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Abstract

Anadara granosa (blood cockles), also known as kerang is a very popular seafood in Malaysia. In 2015, there has been a drastic reduction in the harvest and one of the main reasons is due to the deteriorating water quality in the cockles' breeding environment. Thus, these cockles are exposed and at high risk of being contaminated by pathogenic microorganism because they are filter-feeder organism. Most of the researches on Anadara granosa have focused on only few selected organisms. The overall microbiological assessment of the cockles is lacking therefore this study aimed to determine the types of pathogenic bacteria in blood cockles, Anadara granosa and their antibiotic susceptibility pattern. Thirty pooled sample of Anadara granosa were purchased from 15 wet markets and supermarkets within Klang Valley. All samples were subjected to isolation and identification using standard conventional method. Antibiotic susceptibility test was performed for the different types of bacteria obtained. A total of 85 isolates were successfully isolated and all were gram negative bacteria. Aeromonas spp (23%), Proteus vulgaris (20%) and Vibrio alginolyticus (15%) were the most dominant genus identified. Important human pathogens were also identified which include Vibrio parahaemolyticus (6%), Vibrio cholerae (5%) and Salmonella spp. (2%). All isolates were found to be resistant to Ampicilin (10 μg) and were sensitive to Trimethoprim/sulfamethoxazole (25μg). Among the tested isolates, Aeromonas spp, Klebsiella pneumonia and Vibrio parahaemolyticus were found to be multi-drug resistant. In conclusion, this study suggests that cockles are highly contaminated with pathogenic bacteria and some of the bacteria are multidrug resistant.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	01 Mar 2023 03:14
Last Modified:	01 Mar 2023 03:14
URI:	http://psaspb.upm.edu.my/id/eprint/784

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