Pathogenicity and immunogenicity of fowl adenovirus attenuated in chicken embryo liver cells in specific pathogen free chickens

Azmi, Nurul Ayuni (2017) Pathogenicity and immunogenicity of fowl adenovirus attenuated in chicken embryo liver cells in specific pathogen free chickens. [Project Paper] (Submitted)

Text lp FPV 2017 85.pdf Download (4MB)

Abstract

Fowl adenovirus (FAdV) infection is distributed worldwide and is associated with inclusion body hepatitis (IBH). This disease can cause high mortality rate in flocks of chicken. Thus, it was the objective of this study to determine the pathogenicity and immunogenicity of FAdV isolate (UPM1137) attenuated live in chicken embryo liver (CEL) cells in specific pathogen free (SPF) chickens. Forty five one-day-old SPF chicks were divided into four groups namely A, B, C, and D. Group A, B and C were inoculated with 0.1mL of FAdV passage 15 (P15) with virus titre of 10^5.2 TCID50/0.1mL, 20 (P20) with virus titre of 10^(5.6 )TCID50/0.1mL and 25 (P25) with virus titre of 10^(5.2 )TCID50/0.1mL, respectively through subcutaneous route on days 1 and 14 of age. The group D acted as control group and remain non-inoculated. Sampling was done on days 1, 14 and 28 of age. The chicks were observed at least twice daily for any clinical sign abnormality. Prior to necropsy, blood was collected, and body and liver weight were recorded. Sample of liver was fixed in 10% buffered formalin for histological examination. The study revealed that there were no abnormal clinical signs in all chickens throughout the trial. There were also no significant gross and histological lesions in the liver. The ELISA results showed that FAdV antibody titer was not detected in group D (Control). However, the antibody titer was induced at day 14 in all inoculated group and continued to increase at day 28 of age which is significantly different (p<0.05) when compared to the group D (Control). It was concluded that the live attenuated FAdV in CEL cells is low pathogenic and could induce high FAdV antibody in SPF chickens.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	01 Mar 2023 03:32
Last Modified:	01 Mar 2023 03:32
URI:	http://psaspb.upm.edu.my/id/eprint/789

Actions (login required)

View Item