Effects of Moringa oleifera hydeoethanolic leaf extract on the serum lipid profile in hypercholesterolaemic rats

Loo, Xin Yi (2016) Effects of Moringa oleifera hydeoethanolic leaf extract on the serum lipid profile in hypercholesterolaemic rats. [Project Paper] (Submitted)

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Abstract

Moringa oleifera Lam, (Moringa pterygosperma) also known as ‘drumstick tree’ and ‘horse radish tree’ in India or ‘kelor tree’ in some part of the world is a plant that serve several medicinal and nutritional properties. However, until now, very scanty information is available regarding the anti-hypercholesterolaemic activity of M. oleifera hydroethanolic leaf extract. In this study, the effect of M. oleifera leaf extract was evaluated on hypercholesterolaemic rats induced with alcohol and high-cholesterol diet. A total of 25 male Spague Dawley rats were randomly divided into 5 groups (n=5). Group A as a neutral control receiving normal commercial diet, group B as a negative control, receiving alcohol and high cholesterol diet without treatment, groups C, D, and E as the treatment group, receiving alcohol and high cholesterol diet, concurrent with the M. oleifera hydroethanolic extract at the low, medium and high dose, respectively. At the end of the experiment, blood and liver were collected to measure the serum and liver lipid profile. Liver lipid extraction was done following an adaptation of the published Bligh and Dyer method. Result showed that the relative liver weight of group B (4.11 ± 0.35) was significantly higher compared to group A (3.32±0.46), confirming the negative effect of the alcohol and high cholesterol diet experiment. The 27 days of treatment also significantly reduced kidney fat of groups C and D, compared to group B. The serum low density lipoprotein (LDL) levels of groups C (0.94±0.17), D (0.65±0.41), E (1.04±0.52) were significantly (p<0.05) lower compared to group B (1.25±0.05). The extract also significantly (p<0.05) reduced the atherosclerotic index induced by alcohol and high cholesterol diet as seen in groups C (0.27±0.04), D (0.30±0.06), E (0.36±0.07) as compared to group B (0.41±0.02). However, there was no significant dose effect observed between the treatment groups of different dosages. In conclusion, administration of M. oleifera hydroethanolic extract for 27 days significantly reduced hepatomegaly by decreasing hepatic triglyceride, reduced renal rat, serum LDL and atherosclerotic index. These results suggest that M. oleifera hydroethanolic extract has hepatic-protective properties in hypercholesterolaemia rats.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	07 Mar 2023 04:35
Last Modified:	07 Mar 2023 04:35
URI:	http://psaspb.upm.edu.my/id/eprint/916

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