Isolation and characterization of lactic acid bacteria isolates from deer soil in Universiti Putra Malaysia Bintulu Campus

Wan Mustaffa, Wan Nur Syafawani (2018) Isolation and characterization of lactic acid bacteria isolates from deer soil in Universiti Putra Malaysia Bintulu Campus. [Project Paper] (Submitted)

Text lp FSPM 2018 1 - WAN NUR SYAFAWANI WAN MUSTAFFA.pdf Download (4MB)

Abstract

lsolation and characterization of lactic acid bacteria (LAB) from soil is crucial study as the study of soil are still scarce. The objectives of study are to isolate and characterize potential probiotic and ecological distribution of LAB from deer farm. Ten soil samples were collected randomly from deer farm of Universiti Putra Malaysia, Bintulu. The samples were processed and analysed for total bacteria count and soil acidity. Isolation and characterization of bacteria were done with biochemical test and (GTG)5 PCR fingerprinting. Probiotic analysis (glucose fermentation and bile salt tolerance) were tested on LAB isolates. Data were analysed using Statistical Analysis Software (SAS) software. Total of 11 bacteria were survived from all the probiotic analysis tests (bile salt 0.2%, 1%, 2% and 3% and glucose fermentation) after being selected from morphological of LAB such as gram-positive, catalase-negative and non-motile bacteria are selected. There were 25 cluster of LAB formed. The results obtained in the study confirm the strain viability in bile salt tolerance and sugar fermentation in the selection of potentially probiotic bacteria. These potential probiotic LAB isolated from unusual sample provides more alternative uses in the food industry and nation health in the future.

Item Type:	Project Paper
Subjects:	Q Science > QR Microbiology S Agriculture > S Agriculture (General)
Faculty:	Faculty of Agriculture and Food Sciences
Depositing User:	Mr. Azman Mohamad
Date Deposited:	08 Dec 2025 08:23
Last Modified:	08 Dec 2025 08:23
URI:	http://psaspb.upm.edu.my/id/eprint/2560

Actions (login required)

View Item