Detection of African swine fever virus on flies in selected pig farms in Peninsular Malaysia

Lau, Shu Xin (2023) Detection of African swine fever virus on flies in selected pig farms in Peninsular Malaysia. [Project Paper] (Submitted)

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Abstract

African swine fever (ASF) is a fatal viral disease that causes significant economic losses in the swine industry worldwide due to its high morbidity and mortality. It is caused by African swine fever virus (ASFV) which affects both wild boars and domestic pigs. Transmission route of ASFV have been widely investigated to implement relevant preventive and control measures in reducing the risk of ASF in pig farms. Aside from contaminated fomites and/or infected pigs as infections sources, other possible mechanical vectors such as flies, rats or birds are not well known. This study assessed the detection of ASFV on flies and the different densities of flies in different locations within the farm. Flies from sewage pool, feed house, finisher pens and sow pens of four pig farms in Peninsular Malaysia were collected using sticky fly traps over 24 hours. Five flies of the same genus from each location were pooled together with the head and body separated. Real-time PCR was conducted to detect the presence of ASF DNA virus in the flies. In this study, a total of 2254 flies consisting of Musca sp. (98%), Chrysomya sp. (1%) and Stomoxys sp. (1%) were sampled. Four pooled samples of Musca sp. consisting of 50% head and 50% body were positive for ASFV among 50 total pooled samples. There was no significant difference in densities of flies in the four locations inside the farms. In conclusion, the detection of ASFV in flies indicates that flies are mechanical vectors of ASFV. The high number of flies collected from the farms also highlights the importance of fly control as one of the preventive measures in the transmission of ASF in pig farms.

Item Type:	Project Paper
Faculty:	Faculty of Veterinary Medicine
Depositing User:	Ms. Nordeena Abdul Aziz
Date Deposited:	15 Nov 2024 01:30
Last Modified:	15 Nov 2024 01:30
URI:	http://psaspb.upm.edu.my/id/eprint/2113

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