Zakaria, Nursyahda (2009) Isolation and some characteristics of oil palm bacterial diazotrophs and its plant growth promoting activity. [Project Paper] (Submitted)
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Abstract
Nitrogen fixation carried out by associative and free-living microorganisms in the rhizosphere of plants which has been recognized to play an important role in nitrogen nutrition of plants. Isolation of diazotrophic bacteria from oil palm root and determination of the effects of rhizobacterial inoculation on shallots for its growthpromoting activity under controlled condition were conducted. Out of 107 bacteria which were isolated from rhizosperic soil, rhizoplane and endophyte of oil palm roots, only 10 bacteria showed the ability to fix nitrogen. Growth on N-Free media, Pellicle Formation Test, and tolerance to different pH and salinity was done to test the inoculants obtained from isolation. The ability of the bacteria to grow in nitrogen free broth media is determined by the capability of the isolates to fix nitrogen. Present study showed from ten isolates which have ability to fix nitrogen, five strains were identified as Gram negative while the other five strains were Gram positive. 70% out of ten isolates showed microaerophilic ability. At pH 4, there were no growths of all isolates. Otherwise in pH 6 and 8, all of ten isolates showing strong growth. Strain OP19, OP35, OP44, OP68, OP95, and OP107 were tolerated with all level of NaCL concentrations (0, 0.2, 0.4 and 0.6 M). However, growth of isolate OP22, OP24, OP32 and OP82 were inhibited by the NaCl at the concentrations of 0.4 to 0.6 M. Strains OP22, OP24, OP68 and OP82 showed the most effective in stimulating plant development on root length, shoot height, root number, root fresh and dry weight, and shoot fresh and dry weight.
| Item Type: | Project Paper |
|---|---|
| Subjects: | S Agriculture > S Agriculture (General) S Agriculture > SB Plant culture |
| Faculty: | Faculty of Agriculture and Food Sciences |
| Depositing User: | Mr. Azman Mohamad |
| Date Deposited: | 08 Dec 2025 07:49 |
| Last Modified: | 08 Dec 2025 07:49 |
| URI: | http://psaspb.upm.edu.my/id/eprint/2621 |
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