Sago effluent treatment with Bacillus spp.

Hani, Nurul Wahida (2009) Sago effluent treatment with Bacillus spp. [Project Paper] (Submitted)

Text lpFSPM2009 95 - NURUL WAHIDA BT HANI.pdf Download (3MB)

Abstract

Sago effluent treatment by selective bacteria was conducted to study the effect of isolated bacteria in reducing polluting parameter of the effluent. Starch degrading bacteria were isolated from fresh sago effluent. BI and B2 isolates were found as Bacillus koreensis and Bacillus spp. based on the 16S rDNA sequence analysis. Both bacteria were found efficient in starch degradation at 1.62 and 0.62 mm diameter of clear zone respectively. Four treatments with three replications were carried out in Completely Randomized Design. Each treatment consisted1500 mL effluent which was aerated for ten days. Treatment 2, 3 and 4 were pre-set at pH 6.5 to 7.0. Treatment 2 (control) consisted of effluent mixed with 3 mL of NB whilst Treatment 3 and 4 consisted of effluent with 3 mL of NB suspension cultured with B. koreensis and Bacillus spp. respectively. After 24 hours of treatment, Treatment 3 and 4 showed significant difference in BOD and COD removal. However, reduction of BOD and COD in Treatment 3 was 38 and 25 mg/Lor 14.9 and 9.8% higher than in Treatment 4. Reduction of BOD in Treatment 3 was from 255 to 2 I 7 mg/L whereas from 255 to 230 mg/Lin Treatment 4. For COD removal, as much as 12.7 and 11.3% COD was reduced in Treatment 3 and 4 respectively. The value reduced from 473 to 413 mg/Lin Treatment 3 and from 479 to 425 mg/Lin Treatment 4. In contrast, TSS and pH were found increased in both treatments. Increment of TSS value in Treatment 3 was from 788 to 930 mg/L (15%) and from 794 to 1453 mg/L (45%) in Treatment 4. The pH value increased from pH 7 to 8. As the conclusion, both Bacillus spp. isolated has the ability to reduce sago effluent's physico-chemical characteristics through aeration treatment between O to 24 hours with an exception to TSS. This could be attributed to the adaptability of the bacteria to the new environment, lack of dissolved oxygen at optimum level, lack of nutrients to stimulate bacterial metabolism and degradation activity, short treatment period and others.

Item Type:	Project Paper
Subjects:	S Agriculture > S Agriculture (General)
Faculty:	Faculty of Agriculture and Food Sciences
Depositing User:	Mr. Azman Mohamad
Date Deposited:	08 Dec 2025 07:54
Last Modified:	08 Dec 2025 07:54
URI:	http://psaspb.upm.edu.my/id/eprint/2637

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