Extraction and isolation of chemical compounds from leaves of Ziziphus mauritiana

Daniel, Thierry Henry (2025) Extraction and isolation of chemical compounds from leaves of Ziziphus mauritiana. [Project Paper] (Submitted)

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Abstract

Ziziphus mauritiana considered as medicinal plants with various therapeutic properties and biological activities toward chronic ailments such as cancer, diabetes, and asthma, and other inflammatory conditions. It has antioxidant properties to protect crop against damage induced by pesticides. Realizing the difficulty to isolate the secondary metabolites from the different crude polarities of the leaves raise toward the idea of this work. This work aims to isolate the secondary metabolites and determine the molecular structure from the leaves Z. mauritiana. The extraction of the crude was conducted using different polarities of solvents and followed by separation technique such as thin layer chromatography (TLC) and column chromatography (CC). The isolated compound was analyzed using nuclear magnetic resonance (NMR) spectroscopy to determine the chemical structure. ¹H NMR spectra are compared with the literature review to confirm the molecular structures. The hexane, ethyl acetate, and ethanol crudes obtained at 0.945 g, 0.864 g, and 0.852 g, respectively. Two compounds were isolated from hexane crude (non-polar) known as geranylacetone, and linoleic acid at 0.16 g, and 0.163 g respectively. Two compounds were isolated from ethanol crude (polar) known as 6,10,14-trimethylpentadecan-2-one, and heptadecane at 0.214 g, and 0.410 g, respectively. All of the compounds are consistent with the phytochemical screening of the crudes. All of the compounds were proven their antimicrobial, therapeutic, antitoxicity properties based on the literature review reported. This study contributes the extraction and isolation methodologies with the amount of secondary metabolite. All of these compounds are subjected to the future works related to the various applications that not yet reported.

Item Type:	Project Paper
Subjects:	Q Science > QD Chemistry
Faculty:	Faculty of Humanities, Management and Science
Depositing User:	Mr. Azman Mohamad
Date Deposited:	08 Dec 2025 07:57
Last Modified:	08 Dec 2025 07:57
URI:	http://psaspb.upm.edu.my/id/eprint/2644

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